P‐25 Sensitivity of deep skin scrapings, hair pluckings and exudate microscopy in the diagnosis of canine demodicosis
Identifieur interne : 008A45 ( Main/Exploration ); précédent : 008A44; suivant : 008A46P‐25 Sensitivity of deep skin scrapings, hair pluckings and exudate microscopy in the diagnosis of canine demodicosis
Auteurs : M. N. Saridomichelakis [Grèce] ; A. F. Koutinas [Grèce] ; R. Farmaki [Grèce] ; L. Leontides [Grèce]Source :
- Veterinary Dermatology [ 0959-4493 ] ; 2004-08.
Abstract
Diagnosis of canine demodicosis is mainly based on mite detection in deep skin scrapings (DSS). Hair pluckings (HP) are also helpful, whereas the diagnostic value of exudate microscopy (EM) has never been assessed. The objective of this study was to compare the diagnostic sensitivity of DSS, HP and EM in the various clinical forms of canine demodicosis. A total of 68 dogs with localized (45.6%) or generalized (54.4%) demodicosis, juvenile‐onset (92.6%) or adult‐onset (7.4%) demodicosis were included. Pyoderma was present on 41.2% of cases and absent in 58.8% of cases. One lesion per dog was selected for obtaining one HP, DSS and EM sample, the latter only in 14 cases demonstrating visible exudate. Mineral oil DSS and EM microscopy was focused on a 2.2 × 2.2 mm area, while a total of 100 hair shafts were evaluated by trichogram. At least one parasite was seen in 83.8% of HP, 98.5% of DSS and 100% of EM slides. The sensitivity of HP was higher in the generalized (P = 0.001) and pyoderma‐affected (P = 0.021) cases compared to the localized and noninfected cases. A higher sensitivity of DSS compared to HP was demonstrated in the localized (P = 0.004) and noninfected (P = 0.002) cases. In conclusion, HP should be viewed as a useful diagnostic tool in generalized and infected canine demodicosis, while EM is useful in all deep pyoderma‐associated cases. Funding: Self‐funded.
Url:
DOI: 10.1111/j.1365-3164.2004.00414_25.x
Affiliations:
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Sensitivity of deep skin scrapings, hair pluckings and exudate microscopy in the diagnosis of canine demodicosis</title><author><name sortKey="Saridomichelakis, M N" sort="Saridomichelakis, M N" uniqKey="Saridomichelakis M" first="M. N." last="Saridomichelakis">M. N. Saridomichelakis</name><affiliation wicri:level="1"><country xml:lang="fr">Grèce</country><wicri:regionArea>School of Veterinary Medicine, Aristotles University of Thessaloniki, Greece; School of Veterinary Medicine, University of Thessaly, Karditsa</wicri:regionArea><wicri:noRegion>Karditsa</wicri:noRegion></affiliation></author><author><name sortKey="Koutinas, A F" sort="Koutinas, A F" uniqKey="Koutinas A" first="A. F." last="Koutinas">A. F. Koutinas</name><affiliation wicri:level="1"><country xml:lang="fr">Grèce</country><wicri:regionArea>School of Veterinary Medicine, Aristotles University of Thessaloniki, Greece; School of Veterinary Medicine, University of Thessaly, Karditsa</wicri:regionArea><wicri:noRegion>Karditsa</wicri:noRegion></affiliation></author><author><name sortKey="Farmaki, R" sort="Farmaki, R" uniqKey="Farmaki R" first="R." last="Farmaki">R. Farmaki</name><affiliation wicri:level="1"><country xml:lang="fr">Grèce</country><wicri:regionArea>School of Veterinary Medicine, Aristotles University of Thessaloniki, Greece; School of Veterinary Medicine, University of Thessaly, Karditsa</wicri:regionArea><wicri:noRegion>Karditsa</wicri:noRegion></affiliation></author><author><name sortKey="Leontides, L" sort="Leontides, L" uniqKey="Leontides L" first="L." last="Leontides">L. Leontides</name><affiliation wicri:level="1"><country xml:lang="fr">Grèce</country><wicri:regionArea>School of Veterinary Medicine, Aristotles University of Thessaloniki, Greece; School of Veterinary Medicine, University of Thessaly, Karditsa</wicri:regionArea><wicri:noRegion>Karditsa</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Veterinary Dermatology</title><title level="j" type="alt">VETERINARY DERMATOLOGY</title><idno type="ISSN">0959-4493</idno><idno type="eISSN">1365-3164</idno><imprint><biblScope unit="vol">15</biblScope><biblScope unit="page" from="48">48</biblScope><biblScope unit="page" to="48">48</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08">2004-08</date></imprint><idno type="ISSN">0959-4493</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-4493</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Diagnosis of canine demodicosis is mainly based on mite detection in deep skin scrapings (DSS). Hair pluckings (HP) are also helpful, whereas the diagnostic value of exudate microscopy (EM) has never been assessed. The objective of this study was to compare the diagnostic sensitivity of DSS, HP and EM in the various clinical forms of canine demodicosis. A total of 68 dogs with localized (45.6%) or generalized (54.4%) demodicosis, juvenile‐onset (92.6%) or adult‐onset (7.4%) demodicosis were included. Pyoderma was present on 41.2% of cases and absent in 58.8% of cases. One lesion per dog was selected for obtaining one HP, DSS and EM sample, the latter only in 14 cases demonstrating visible exudate. Mineral oil DSS and EM microscopy was focused on a 2.2 × 2.2 mm area, while a total of 100 hair shafts were evaluated by trichogram. At least one parasite was seen in 83.8% of HP, 98.5% of DSS and 100% of EM slides. The sensitivity of HP was higher in the generalized (P = 0.001) and pyoderma‐affected (P = 0.021) cases compared to the localized and noninfected cases. A higher sensitivity of DSS compared to HP was demonstrated in the localized (P = 0.004) and noninfected (P = 0.002) cases. In conclusion, HP should be viewed as a useful diagnostic tool in generalized and infected canine demodicosis, while EM is useful in all deep pyoderma‐associated cases. Funding: Self‐funded.</div></front></TEI><affiliations><list><country><li>Grèce</li></country></list><tree><country name="Grèce"><noRegion><name sortKey="Saridomichelakis, M N" sort="Saridomichelakis, M N" uniqKey="Saridomichelakis M" first="M. N." last="Saridomichelakis">M. N. Saridomichelakis</name></noRegion><name sortKey="Farmaki, R" sort="Farmaki, R" uniqKey="Farmaki R" first="R." last="Farmaki">R. Farmaki</name><name sortKey="Koutinas, A F" sort="Koutinas, A F" uniqKey="Koutinas A" first="A. F." last="Koutinas">A. F. Koutinas</name><name sortKey="Leontides, L" sort="Leontides, L" uniqKey="Leontides L" first="L." last="Leontides">L. Leontides</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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